Repressive effect of miRNA-363 via SOX4 on human osteosarcoma cell growth and apoptosis

AIM: To detect the expression of miRNA-363 and SOX4 in osteosarcoma tissues and to investigate the effect of miRNA-363 on the viability and apoptosis of human osteosarcoma cell line MG-63.METHODS: Real-time PCR was used to detect the expression level and the relationship of miRNA-363 and SOX4 mRNA in the osteosarcoma tissues and the corresponding paratumorous tissues collected from 63 patients. The expression levels of miRNA-363 and SOX4 in osteosarcoma cell line MG-63 after transfected with miRNA-363 mimics were measured. The cell viability was measured by CCK-8 assay. Flow cytometry was used to monitor the changes of cell cycle and apoptosis. The changes of SOX4 and miRNA-363 expression levels in the MG-63 cells after transfection with SOX4 siRNA or pcDNA/SOX4 was detect by real-time PCR.RESULTS: The expressed level of miRNA-363 was lower, and the expression level of SOX4 was higher in the osteosarcoma tissues than those in the adjacent normal tissues. A significantly negative correlation between the expression levels of miRNA-363 and SOX4 was observed. The expression of miRNA-363 in the MG-63 cells after transfection with miRNA-363 mimics was significantly up-regulated, while the expression of SOX4 in the MG-63 cells was significantly down-regulated, with significant difference as compared with the cells transfected with miRNA-NC and control cells. The viability of MG-63 cells was inhibited, the cell cycle was arrested in G₀/G₁ phase, and the cell apoptosis was increased by transfection with miRNA-363 mimics. The relative protein expression levels of SOX4 in SOX4 siRNA group and pcNDA/SOX4 group were significantly different from those in negative control group, but the relative expression levels of miRNA-363 had no significant difference. Over-expression of SOX4 restored the viability of the MG-63 cells reduced by miR-363.CONCLUSION: The expression level of miRNA-363 is low in human osteosarcoma tissue. miRNA-363 may inhibits the viability of osteosarcoma cell line MG-63 and promotes cell apoptosis in vitro via inhibiting the SOX4 expression.     

Determination of immunochemically nonreactive urinary albumin by HPLC method and its significance of diagnose on children diabetic nephropathy

AIM: To detect the immunochemically nonreactive urinary albumin and its significance of diagnose on diabetic nephropathy by high performance liquid chromatography (HPLC).METHODS: Urine samples of 58 diabetic children were collected. Urinary albumin was measured by HPLC and by immunoassay respectively.RESULTS: The retention times of albumin of standard and urine samples were 14.3 min. The content of albumin by HPLC and by immunoassay were 9.6-195.1 mg/L and 0-158.6 mg/L, respectively.The level of urinary albumin was higher by HPLC detection than that by immunoassay (P<0.05) in the same sample. The microalbuminuria rates were 70.7% detected by HPLC and 37.9% by immunoassay.CONCLUSION: Total urinary albumin can be measured by HPLC. Microalbuminuria in diabetic children can be observed earlier by HPLC.     

香石竹快速繁殖技术研究

以中花散枝香石竹"红芭芭拉"的茎段为外植体,探讨不同培养基对香石竹的诱芽萌动、继代增殖和生根培养的影响。结果表明:外植体切取部位的诱导芽萌动率从高至低排序为:顶芽带腋芽的茎段不带腋芽的茎段;当培养基为MS+6-BA 1.0mg/L时,对香石竹带腋芽的茎段芽萌动最好;在培养基为1/2MS+6-BA 1.0mg/L+NAA 0.2mg/L时,继代增殖长芽的效果最好;在培养基为1/2MS+6-BA 0.2mg/L+NAA 0.5mg/L时,诱导不定根效果最好。     

采伐联合机液压驱动控制系统研究

介绍了采伐联合机的基本结构和工作原理,建立了液压驱动控制系统数学模型,为进一步研究机械手的驱动控制系统奠定了基础.     

ZnO/Na 2 O(mol)对cBN陶瓷砂轮性能的影响

通过DSC -TG综合热分析、X衍射分析、线膨胀测试仪、扫描电镜等测试方法研究了ZnO/Na 2 O(mol)（ Z 值）对Li 2 O -K 2 O -SiO 2 -Al 2 O 3 -B 2 O 3 系陶瓷结合剂及立方氮化硼砂轮性能的影响，结果发现：ZnO能够抑制α -石英晶相析出，诱导析出低膨胀晶体Li 2 OAl 2 O 3 7.5SiO 2 ，并随着 Z 值的增加，α -石英减少，Li 2 OAl 2 O 3 7.5SiO 2 晶体逐渐增多， Li 2 OAl 2 O 3 7.5SiO 2 晶型结构趋于完整，晶粒尺寸减小，结构更加致密；当砂轮配方一定时，砂轮的体积密度、抗弯曲强度、洛氏硬度均随着 Z 值的增加而增大，气孔率减小，磨削（干磨）铸铁（QT400）时磨耗比先增加后减小；当 Z =0.75时，结合剂的膨胀系数为6.902 9×10 -6 K - 1 ，耐火度为805 ℃，砂轮的烧结温度为845 ℃，抗弯曲强度为73.97 MPa，气孔率为15.65%，磨耗比最大为156.42%.     

酸樱桃新品种‘玫蕾’

 ‘玫蕾’是从野生酸樱桃实生选种而来。单果质量5.3 g，总糖含量8.92%，总酸度1.49%，可溶性蛋白质含量1.46%，维生素C含量0.152 mg · g^-1 FW，铁含量0.040 mg · g^-1 FW，钙含量0.914 mg · g^-1FW。乔木，6年树冠高达3.6 m，果实表面颜色为紫红色，有光泽，果实甜酸，酸味浓郁，汁多，果肉深红色。适宜陕西关中地区栽植。     

路易斯安娜鸢尾组培工厂化生产技术研究

研究了路易斯安娜鸢尾组培工厂化生产技术。结果表明:工厂化苗木生产主要采用抑菌剂结合传统消毒方法进行脱毒、试管增殖、田间扩繁相结合的方法,壮苗期间同时生根,简化了组培程序,缩短了育苗周期,降低了生产成本。苗木移栽成活率达到98%以上,每年可生产优质无菌鸢尾苗30万株。     

咸安苎麻产业化发展的若干思考

本文分析了湖北咸宁市咸安区苎麻产业的发展现状和机遇,以及发展苎麻生产的优势.指出了咸安苎麻产业所面临的各种挑战,并提出了相关发展对策.     

桂西北地区杉木冰冻雪灾与恢复措施

2008年1月中旬以来我国南方大部分省区遭受了历史罕见的雨雪冰冻灾害,使广西的森林资源和林业基础设施遭受惨重损失。针对广西西北部地区杉木(Cunninghamia laceolata)的受害类型和严重程度,结合杉木的生长特性和实际生产需要,提出相应的恢复技术。